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Primadex tablet fungsi, and an equal volume of ethyl acetate were added to the anaerobic phase. samples were then incubated at 37° C. in the dark at 2% CO 2 for 48 h. The samples were then decanted off and concentrated by vacuum filtration. The fractions were then dried under a stream of nitrogen using vacuum system with a regulator to remove remaining impurities. The extract was chromatographed on a TLC plate. Bifurcating microsomes Bifurcating microsomes had previously been reported to allow for the selective isolation of DNA with a high purity, while the selective extraction of RNA and protein [28]. Bifurcating microsomes differ from conventional in that the DNA and protein were separated in separate wells and the RNA protein were simultaneously extracted from the two wells. To facilitate extraction of DNA and protein with a high purity, an equal volume of ethyl acetate was added canada pharmacy generic cialis to the anaerobic phase. samples were incubated at 37° C. in the dark at 2% CO 2 for 48 h. The samples were then decanted off and concentrated by vacuum filtration. The fractions were then dried under a stream of nitrogen using vacuum system with a regulator to remove remaining impurities. The extracts were chromatographed on a TLC plate. Statistical analysis The data were expressed in mean±standard error (SE). For statistical analysis, the data were analyzed with use of two-way ANOVA followed by Tukey's post test. Results are mean±standard error (SE) unless otherwise indicated. The level of significance was adjusted using the Bonferroni's post hoc test: P<0.05 was considered to be statistically significant. Results and discussion In brief, our results indicate that there is a significant difference in the relative abundance of DNA and protein in the two samples, with higher levels of DNA in the samples two individuals tested. However, there was no significant difference in their relative abundance of the two lipoproteins, except for mRNA level of total cholesterol, which was significantly higher in the sample of older individual. Total cholesterol was measured by a highly specific kit and was within the standard range for this assay (see Table 1). For all three of the lipoproteins tested, levels were significantly higher in the samples of older group than in those of the younger group, except for protein level of HDL cholesterol which was significantly higher in the older group. Table 1. Lipoprotein (A) Total cholesterol (mg/dL) HDL Triglycerides cholesterol* Triglycerides* Young 30.4 2.4 2.1 1.3 1.13 Older 28.7 1.6 1.5 0.9 0.95 *P<0.01. Source: Stegle et al. (2000). The analysis of mRNA level total cholesterol demonstrated that the mRNA expression level of total cholesterol in this sample was significantly higher in the older group. mRNA expression level of HDL cholesterol was also significantly higher in the older group. Furthermore, there was a significant difference in mRNA expression level for total cholesterol, HDL and triglycerides. There was no significant difference in total cholesterol, HDL or triglycerides between the two cohorts (Figure 1). There was a significant difference in the mRNA expression level of total cholesterol between the two individuals tested in older individual. Figure 1. View largeDownload slide The mRNA expression level of total cholesterol in the two samples. Concerning the mRNA expression levels of three lipoproteins, we have also measured the mRNA expression levels of total cholesterol, HDL and triglycerides in other samples of these two cohorts. In the samples of younger and older individuals, the mRNA expression levels and of the three lipoproteins were all within the standard range (Table 2). However, the levels of total cholesterol, HDL and triglycerides in the samples of older individual were significantly higher than in the younger individual. This was not a new observation; it is consistent with the previous study of Stegle et al. (2000) [5]. However, the mRNA expression level of total cholesterol was significantly higher in this older individual than the younger individual. This was an unexpected finding, and we do not understand the mechanisms behind this difference. Table 2. Sample Young age group (years) Older Total cholesterol:mg/dL HDL-cholesterol:mg/dL Triglycerides:mg/dL HDL-cholesterol*:mg/dL Triglycerides*:mg/dL Total cholesterol:mg/dL HDL-ch.

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